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fap rnascope ish assay  (Advanced Cell Diagnostics Inc)


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    Structured Review

    Advanced Cell Diagnostics Inc fap rnascope ish assay
    The tumour-immune phenotypes for a vendor-procured collection (including both primary tumours and recurrent tumours, n = 84 samples) were predicted based on gene expression. The pattern of CD8 + T-cell infiltration and molecular features associated with excluded tumours were validated using immunohistochemistry and in situ hybridization <t>(ISH)</t> on FFPE tumour tissues. a Representative images of CD8 IHC (top), MHC-I-IHC (middle) and <t>FAP</t> ISH (bottom) are shown for the three tumour-immune phenotypes. b Percentage of CD8 staining over tumour/stroma area ( n = 72 samples), H scores for MHC-I ( n = 77 samples) and FAP expression in the tumour or the stroma ( n = 77 samples) were presented by the three-class tumour-immune phenotypes. c RNA-seq gene expression level, represented as Log 2 (RPKM+1) for CD8A , HLA-A and FAP , is presented across the three-class tumour-immune phenotypes. b , c Whiskers ranging from minima to maxima, median and 25–75% IQR shown by boxplots; each dot is a tumour sample (primary tumours and recurrent tumours are pooled). The statistical significance is displayed with the exact P values on the graphs and calculated with a Kruskal–Wallis test corrected for multiple comparisons (Dunn’s test). Source data are provided as a Source Data file.
    Fap Rnascope Ish Assay, supplied by Advanced Cell Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fap+rnascope+ish+assay/fap+rnascope+ish+assay/pmc07642433-251-0-11
    Average 90 stars, based on 1 article reviews
    fap rnascope ish assay - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Integrated digital pathology and transcriptome analysis identifies molecular mediators of T-cell exclusion in ovarian cancer"

    Article Title: Integrated digital pathology and transcriptome analysis identifies molecular mediators of T-cell exclusion in ovarian cancer

    Journal: Nature Communications

    doi: 10.1038/s41467-020-19408-2

    The tumour-immune phenotypes for a vendor-procured collection (including both primary tumours and recurrent tumours, n = 84 samples) were predicted based on gene expression. The pattern of CD8 + T-cell infiltration and molecular features associated with excluded tumours were validated using immunohistochemistry and in situ hybridization (ISH) on FFPE tumour tissues. a Representative images of CD8 IHC (top), MHC-I-IHC (middle) and FAP ISH (bottom) are shown for the three tumour-immune phenotypes. b Percentage of CD8 staining over tumour/stroma area ( n = 72 samples), H scores for MHC-I ( n = 77 samples) and FAP expression in the tumour or the stroma ( n = 77 samples) were presented by the three-class tumour-immune phenotypes. c RNA-seq gene expression level, represented as Log 2 (RPKM+1) for CD8A , HLA-A and FAP , is presented across the three-class tumour-immune phenotypes. b , c Whiskers ranging from minima to maxima, median and 25–75% IQR shown by boxplots; each dot is a tumour sample (primary tumours and recurrent tumours are pooled). The statistical significance is displayed with the exact P values on the graphs and calculated with a Kruskal–Wallis test corrected for multiple comparisons (Dunn’s test). Source data are provided as a Source Data file.
    Figure Legend Snippet: The tumour-immune phenotypes for a vendor-procured collection (including both primary tumours and recurrent tumours, n = 84 samples) were predicted based on gene expression. The pattern of CD8 + T-cell infiltration and molecular features associated with excluded tumours were validated using immunohistochemistry and in situ hybridization (ISH) on FFPE tumour tissues. a Representative images of CD8 IHC (top), MHC-I-IHC (middle) and FAP ISH (bottom) are shown for the three tumour-immune phenotypes. b Percentage of CD8 staining over tumour/stroma area ( n = 72 samples), H scores for MHC-I ( n = 77 samples) and FAP expression in the tumour or the stroma ( n = 77 samples) were presented by the three-class tumour-immune phenotypes. c RNA-seq gene expression level, represented as Log 2 (RPKM+1) for CD8A , HLA-A and FAP , is presented across the three-class tumour-immune phenotypes. b , c Whiskers ranging from minima to maxima, median and 25–75% IQR shown by boxplots; each dot is a tumour sample (primary tumours and recurrent tumours are pooled). The statistical significance is displayed with the exact P values on the graphs and calculated with a Kruskal–Wallis test corrected for multiple comparisons (Dunn’s test). Source data are provided as a Source Data file.

    Techniques Used: Gene Expression, Immunohistochemistry, In Situ Hybridization, Staining, Expressing, RNA Sequencing

    Related Articles

    RNAscope:

    Article Title: Integrated digital pathology and transcriptome analysis identifies molecular mediators of T-cell exclusion in ovarian cancer
    Article Snippet: CD8 IHC was performed at Histogenex on Ventana Benchmark using C8/clone 144B anti-CD8a monoclonal antibodies (Agilent Dako, #GA623). .. Single-plex FAP RNAscope ISH assay was designed, implemented and scored at Advanced Cell Diagnostics (Hayward, CA). ..

    In Situ Hybridization:

    Article Title: Integrated digital pathology and transcriptome analysis identifies molecular mediators of T-cell exclusion in ovarian cancer
    Article Snippet: CD8 IHC was performed at Histogenex on Ventana Benchmark using C8/clone 144B anti-CD8a monoclonal antibodies (Agilent Dako, #GA623). .. Single-plex FAP RNAscope ISH assay was designed, implemented and scored at Advanced Cell Diagnostics (Hayward, CA). ..



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    The tumour-immune phenotypes for a vendor-procured collection (including both primary tumours and recurrent tumours, n = 84 samples) were predicted based on gene expression. The pattern of CD8 + T-cell infiltration and molecular features associated with excluded tumours were validated using immunohistochemistry and in situ hybridization <t>(ISH)</t> on FFPE tumour tissues. a Representative images of CD8 IHC (top), MHC-I-IHC (middle) and <t>FAP</t> ISH (bottom) are shown for the three tumour-immune phenotypes. b Percentage of CD8 staining over tumour/stroma area ( n = 72 samples), H scores for MHC-I ( n = 77 samples) and FAP expression in the tumour or the stroma ( n = 77 samples) were presented by the three-class tumour-immune phenotypes. c RNA-seq gene expression level, represented as Log 2 (RPKM+1) for CD8A , HLA-A and FAP , is presented across the three-class tumour-immune phenotypes. b , c Whiskers ranging from minima to maxima, median and 25–75% IQR shown by boxplots; each dot is a tumour sample (primary tumours and recurrent tumours are pooled). The statistical significance is displayed with the exact P values on the graphs and calculated with a Kruskal–Wallis test corrected for multiple comparisons (Dunn’s test). Source data are provided as a Source Data file.
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    The tumour-immune phenotypes for a vendor-procured collection (including both primary tumours and recurrent tumours, n = 84 samples) were predicted based on gene expression. The pattern of CD8 + T-cell infiltration and molecular features associated with excluded tumours were validated using immunohistochemistry and in situ hybridization <t>(ISH)</t> on FFPE tumour tissues. a Representative images of CD8 IHC (top), MHC-I-IHC (middle) and <t>FAP</t> ISH (bottom) are shown for the three tumour-immune phenotypes. b Percentage of CD8 staining over tumour/stroma area ( n = 72 samples), H scores for MHC-I ( n = 77 samples) and FAP expression in the tumour or the stroma ( n = 77 samples) were presented by the three-class tumour-immune phenotypes. c RNA-seq gene expression level, represented as Log 2 (RPKM+1) for CD8A , HLA-A and FAP , is presented across the three-class tumour-immune phenotypes. b , c Whiskers ranging from minima to maxima, median and 25–75% IQR shown by boxplots; each dot is a tumour sample (primary tumours and recurrent tumours are pooled). The statistical significance is displayed with the exact P values on the graphs and calculated with a Kruskal–Wallis test corrected for multiple comparisons (Dunn’s test). Source data are provided as a Source Data file.
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    Image Search Results


    The tumour-immune phenotypes for a vendor-procured collection (including both primary tumours and recurrent tumours, n = 84 samples) were predicted based on gene expression. The pattern of CD8 + T-cell infiltration and molecular features associated with excluded tumours were validated using immunohistochemistry and in situ hybridization (ISH) on FFPE tumour tissues. a Representative images of CD8 IHC (top), MHC-I-IHC (middle) and FAP ISH (bottom) are shown for the three tumour-immune phenotypes. b Percentage of CD8 staining over tumour/stroma area ( n = 72 samples), H scores for MHC-I ( n = 77 samples) and FAP expression in the tumour or the stroma ( n = 77 samples) were presented by the three-class tumour-immune phenotypes. c RNA-seq gene expression level, represented as Log 2 (RPKM+1) for CD8A , HLA-A and FAP , is presented across the three-class tumour-immune phenotypes. b , c Whiskers ranging from minima to maxima, median and 25–75% IQR shown by boxplots; each dot is a tumour sample (primary tumours and recurrent tumours are pooled). The statistical significance is displayed with the exact P values on the graphs and calculated with a Kruskal–Wallis test corrected for multiple comparisons (Dunn’s test). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Integrated digital pathology and transcriptome analysis identifies molecular mediators of T-cell exclusion in ovarian cancer

    doi: 10.1038/s41467-020-19408-2

    Figure Lengend Snippet: The tumour-immune phenotypes for a vendor-procured collection (including both primary tumours and recurrent tumours, n = 84 samples) were predicted based on gene expression. The pattern of CD8 + T-cell infiltration and molecular features associated with excluded tumours were validated using immunohistochemistry and in situ hybridization (ISH) on FFPE tumour tissues. a Representative images of CD8 IHC (top), MHC-I-IHC (middle) and FAP ISH (bottom) are shown for the three tumour-immune phenotypes. b Percentage of CD8 staining over tumour/stroma area ( n = 72 samples), H scores for MHC-I ( n = 77 samples) and FAP expression in the tumour or the stroma ( n = 77 samples) were presented by the three-class tumour-immune phenotypes. c RNA-seq gene expression level, represented as Log 2 (RPKM+1) for CD8A , HLA-A and FAP , is presented across the three-class tumour-immune phenotypes. b , c Whiskers ranging from minima to maxima, median and 25–75% IQR shown by boxplots; each dot is a tumour sample (primary tumours and recurrent tumours are pooled). The statistical significance is displayed with the exact P values on the graphs and calculated with a Kruskal–Wallis test corrected for multiple comparisons (Dunn’s test). Source data are provided as a Source Data file.

    Article Snippet: Single-plex FAP RNAscope ISH assay was designed, implemented and scored at Advanced Cell Diagnostics (Hayward, CA).

    Techniques: Gene Expression, Immunohistochemistry, In Situ Hybridization, Staining, Expressing, RNA Sequencing